Analysis of PheA aminoacyl synthetase.

Analysis performed on 2024-07-12 13:54:37

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 87463 1738 85725 27044
Archaea 6380 153 6227 1414
Vertebrata 2094 657 1437 454
Mammals 1049 173 876 129

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
508 15 453

Domains

Domains
Current Study No Domains Found
Guo et al (2010) No Domains Listed

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 508 0 508 Undefined
Number of Variants 468 0 468 Undefined
Number of Pathogenic Variants 15 0 15 Undefined
Number of Benign/Unknown Variants 453 0 453 Undefined
Fisher exact test Undefined

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 508 0 508 Undefined
Number of Variants 468 0 468 Undefined
Number of Pathogenic Variants 15 0 15 Undefined
Number of Benign/Unknown Variants 453 0 453 Undefined
Fisher exact test Undefined

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 508 0 0.80 (0.01) nan (nan) 7.724e-01 NaN Undefined Undefined
Guo et al (2010) 508 0 0.80 (0.01) nan (nan) 7.724e-01 NaN Undefined Undefined

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains